During this period, researchers unified chemical, anatomical, and electrophysiological approaches to the peripheral nervous system, foregrounding acetylcholine as the chemical mediator of neuromuscular transmission. Foundational neuroanatomical mapping detailed motor endplates and peripheral innervation, while innovative histological staining methods enabled visualization of nerve fibers in paraffin sections. Electrophysiological studies of cutaneous sensory nerves advanced early models of peripheral sensation, integrating motor and sensory pathways into a cohesive framework.